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3: J Food Prot 2003 May;66(5):854-9 Related Articles, Links
Improving extraction of fumonisin mycotoxins from Brazilian corn-based
infant foods.
Sewram V, Shephard GS, Marasas WF, Penteado MF, de Castro M.
PROMEC Unit, Medical Research Council, P.O. Box 19070, Tygerberg 7505, South
Africa. vikash.sewram@mrc.ac.za
The current AOAC International methods for the determination of fumonisins have
been validated for corn and cornflakes but have produced low recoveries and high
variability when applied to processed corn products for infants. Hence, an
investigation was undertaken to improve the extraction efficiency for fumonisins
by investigating the use of different extraction solvents. Corn-based infant
foods containing cornmeal, corn starch, and corn flour were purchased in the
city of Campinas, state of Sao Paulo, Brazil, and were analyzed for fumonisins
B1 (FB1), B2 (FB2), and B3 (FB3) following extraction with a range of solvents.
Comparison of the results from each of the samples indicated that acidified 70%
aqueous methanol at pH 4.0 provided the best overall performance, whereas a
methanol/boric acid (pH 9.2) mixture displayed poor extraction efficiency.
Extraction with acidified 70% aqueous methanol showed seven of eight test
samples to be positive for FB1 (range, 30 to 6,127 microg/kg; relative SD, 4.2
to 51.7%), two of eight samples to be positive for FB2 (range, 53 to 1,738
microg/kg; relative SD, 4.5 to 5.3%), and one of eight samples to be positive
for FB3 (575 microg/kg). For samples in which extraction with phosphate-buffered
mixtures (pH 3) proved superior, the method suffered from poor chromatography
due to interfering compounds. The findings indicate that matrix interferences
play a significant role in the extractability, cleanup, and chromatography of
the fumonisins.
PMID: 12747696
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